Sunday 11 July 2010

TPS: blooms, hybrids and berrybags

Pirampo x Khuchi Akita F3
Beautiful purple potato blossom with white star points. This is seedling no.3 of Pirampo x Khuchi Akita.

My potato plants grown from TPS have started flowering, and very beautiful they are too. At the moment it's only the diploid ones which are in bloom, and although the tetraploids are starting to form buds they are a way behind.

I have two diploid lines on the go, both of them grown from hybrid seed produced by Tom Wagner and given to me by Patrick. They are Skagit Valley Gold x Thumbed Nose F1 and Pirampo x Khuchi Akita F3. Actually I also have some diploid plants from Mayan Gold OP seed I collected a couple of years ago, but those were sown a couple of weeks later than the other two and are not flowering yet.

Skagit Valley Gold is one of Tom Wagner's own varieties, derived from Andean potato lines, which makes it genetically distinct from the vast majority of American and European potatoes. It has small round tubers with orange-yellow flesh which cook very quickly, taste very special, and are high in carotenoids and Vitamin E. Thumbed Nose, its partner in this cross, is also one of Tom's own varieties but I can't tell you much about that one. I only have two plants of this hybrid and they are looking extremely unlike each other, although both have the distinctive diploid foliage. One is tall and dark-leaved with pigmentation in the veins, and hasn't flowered yet (though it has buds). The other is a bright lime green and much more compact, with little or no pigment. That one is flowering (pictured below) and the blossoms are an attractive pink mauve.

Skagit Valley Gold x Thumbed Nose with bee
Skagit Valley Gold x Thumbed Nose F1 blossom, providing some entertainment for a bee.

The flowers on diploid potatoes look just the same as flowers on tetraploids (although they seem to have some additional colour options - I've never seen dark purple blossoms on a tetraploid) but they have some practical differences. Maybe it's to do with Tom's diligence in using only the most fertile varieties in his breeding work, but these flowers are producing stupendous amounts of pollen. As you can see in the photo, they are attractive to bees. This is interesting because bees are among the natural pollinators of potatoes but I've very rarely seen them taking an interest in regular tetraploid varieties. So prolific is the pollen production on these blossoms there were plumes of it going up like a puff of talcum powder just from the beating of the bee's wings. Needless to say I've been using the pollen to make some more crosses and a single anther is enough to pollinate pretty much everything I have available. There is just masses of it.

The other thing that's different about diploids is that they're normally self-incompatible. This inability to fertilise their own flowers is genetic, and forces them to outbreed with other potatoes. The first two blossoms on this plant fell off, unfertilised, despite the amount of pollen they were chucking around, as there was nothing else to fertilise them. Now that the Khuchi Akita hybrids are flowering I've been pollinating them with that, so hopefully I will get some berries now.

Pirampo and Khuchi Akita are both rare landraces from Bolivia. I don't know much about them, the only available information being in the records of the United States Department of Agriculture genebank, which lists them both as a "primitive cultivar". My understanding is that they have been cultivated for many generations by Bolivian farmers but have never been commercially available, and are closer to wild potatoes than to modern spuds. The appeal of this is obvious - they have diversity which would simply not be found in so-called "advanced cultivars". They are both of the Stenotomum type - that is, they're what used to be regarded as a separate species called Solanum stenotomum but which is now classified merely as a subspecies or subgroup of the standard Solanum tuberosum. Confused yet? Just look at the pretty flowers!

Pirampo x Khuchi Akita F3
Pirampo x Khuchi Akita F3 blossom. This is plant no.4 in my batch, but they all have fairly similar flowers so far, varying in shades of purple and mauve.

The seeds I was given are described as being an F3. That just means that Tom has recombined the offspring a couple of times since the original cross. They have a lot of diversity, which I'm seeing more clearly because I have a number of plants ... about 15 altogether, of which eight or nine are now flowering. Most of them have flowers similar to the one shown above, a deep rich velvety purple. There are subtle variations in the purple, with some having a magenta hue and others more blue. Some have white tips on the petals. There are also some coming out mauve, again with some variation between pinky-mauve and bluey-mauve. Unfortunately the blossoms tend to be borne facing downwards, so you have to lift them up to see how beautiful they are! The plants are also varied in tallness, vigour, and the amount of colour on the stems and leaves.

I know little about the tuber characteristics of the parent varieties nor what to expect from my plants, but while I was earthing up the other day some soil fell away and I got a glimpse of a pink-skinned tuber under one of the plants, which seems to have set tubers exceptionally early. I'm not talking about the subtle rosy brick pink of a Pink Fir Apple here. I mean this one was really PINK. As in bright, vivid carmine. I left it in situ and covered it back up, but I can't wait to see what other variants these plants come up with.

I have to say, TPS is amazing stuff. The seeds are tiny and the emerging seedlings as fine as cotton threads; they have no tuber to provide them with support or nourishment. And yet in less than three months my TPS-grown plants are as large and voluptuous as most of my tuber-grown plants.

So of course I've been making a lot more hybrids for next year's seed, using various tuber-grown varieties. This berry is on a Sharpe's Express plant, pollinated with Salad Blue.

Sharpe's Express potato berry
The one berry containing (I hope) F1 seeds of Sharpe's Express x Salad Blue.

Sharpe's Express is not an easy variety to make berries with, as it is a very poor flower holder. It produces very pretty flowers when it's in the mood, of a pale lilac mauve colour. But the vast majority of its buds are thrown off before they open, and I only had one cyme of mature buds this year, of which only three flowers opened. I pollinated them all very carefully with pollen from Salad Blue. Two of them fell off before they had a chance to develop into a fruit. But one - just one - successfully set a berry. I go out every day and pray for it to stay on just a little longer and not drop prematurely. Such is the tenuous nature of making hybrids with some varieties.

There are other frustrating varieties too. Negresse, which would be a wonderful variety to breed from, appears not to produce any pollen (is anyone else having any luck with it?) Its anthers are hard and sterile and I can't get anything out of them. Congo is not much better. And my Mr Little's Yetholm Gypsy is declining to flower at all this year, casting off its unopened buds with wilful profligance. But I've also had some successes. Pink Fir Apple was being a pain in the arse, producing masses of flowers but not holding them long enough for a berry to set. Through patience and persistence I've now got quite a lot of berries on it, simply by keeping on pollinating more flowers day after day. Again Salad Blue has been the primary pollinator ... as I'm tempted by the idea of knobbly blue fingerlings. Highland Burgundy Red has given me a pleasant surprise too. I've been growing it for years and it's never once set a berry for me. However I've found its female fertility to be very high - it sets berries very readily when presented with decent pollen. I assumed it was itself male-sterile, but just out of curiosity I made a couple of test crosses, using its pollen to attempt to fertilise other varieties. To my surprise I got a berry. So I tried hand-pollinating it with its own pollen, and again, one or two berries ensued. The pollen fertility doesn't seem to be as high as Salad Blue's, but it certainly can be used as a male parent as well as a female. Perhaps the reason it doesn't naturally pollinate itself is more to do with flower physiology. It has an exceptionally long pistil which sticks way out of the end of the flower, and it also has very small compact anthers. Maybe the distance between the anthers and the stigma is just too great to enable self-pollination without assistance.

So I now have quite a lot of swelling berries on a great many of my different potato plants, all hand-pollinated and marked with colour-coded wool to identify the fathers. However, there is something I will have to watch out for. The berries are quite visible to start with but as they get heavier they tend to sag down into the foliage, and are lost from view. Some sag as far as the ground, where they're vulnerable to being nibbled at or stepped on. They also have a habit of dropping off the plant unexpectedly, which is not a problem in itself as the seeds inside will carry on maturing even when detached from the plant prematurely (within reason), but it does become a problem if there's a chance of them getting lost. If they roll away from the plant it can be difficult to be sure where they came from, and the coloured threads which identify the father varieties are very liable to get detached from them. What I need - at least for those which are most precious or easily lost - is a small and very lightweight bag to hang over the berries as they near maturity, so if they do suddenly decide to make a break for freedom I have them protected and contained. I've read about people using paper bags for this, which are certainly a cheap and convenient solution, but in the British climate that's about as much use as a chocolate teapot (and for similar reasons). They need to be weatherproof, and porous enough to let air and moisture in and out, translucent enough that I can see the berries inside and light enough not to add any extra strain on the stalks, and ideally re-usable. Here's what I came up with.

Potato berry bags
Berrybags. Not the tidiest stitching, but I think these will do the job very nicely.

They are simply made from a scrap of nylon gauze of the type that's sold for making window drapes (hence the pretty gold pattern, but that's optional) and offcuts of this kind of material can be got from fabric shops for next to nothing. I'm simply folding them over and stitching them up by hand, and very roughly, as the aesthetics don't really matter. The top edge has a piece of wool folded into it to make a drawstring, and I sealed the edges with a fray-stopping chemical goo so they don't fall to bits. They take about 15-20 minutes to make and as I'm the kind of person who never measures anything they are in several different sizes, some small enough for a single berry and some big enough for a whole cyme. The bag goes over the berry (including its coloured marker thread, to make sure that stays with the berry) and the drawstring is tied up around the stem.

Potato berry bag

Wednesday 7 July 2010

July miscellany

Papaver rhoeas
Is there anything more beautiful than poppies at this time of year? This is one of my Somme poppies (see below) but with white flecks.

My last couple of posts have been pretty demanding in terms of time and research, so I feel the need just to blather about more casual stuff today.

Tomatoes ... what can I tell you about tomatoes?

I have a greenhouse full of 'em, laden with flowers and green fruits. I've been doing a few crosses, in a half-arsed kind of way, but I consider them very much a sideline. For one thing, I get fed up with hand-pollinating tomatoes very quickly. In principle they are exactly the same as potatoes and all the instructions I gave in my potato-hybridising post should translate conveniently to tomatoes, which are of course from the Solanum family and have the same basic flower type, except that they're small and yellow. But I find tomato pollination much more frustrating. The small flowers are very fiddly to work with, and the anthers tend to be tightly fused into a cone, so you have to separate them with a careful incision ... they can't be succulently and individually plucked like potato anthers. They are quite a bugger to get off, in fact - which wouldn't be a problem if it wasn't for the fact that they are snugly clamped against a ridiculously fragile pistil. There's none of the "green bendy bit" as described in my pea video ... with tomatoes it's an unyielding green brittle bit. I've destroyed flower after flower by accidentally clonking the pistil off as I attempt to wrench at recalcitrant anthers. Some varieties have a pistil so fine and spindly you can barely see it. And when you do find it, the pollen has to be applied so lightly and delicately, because the tiniest shove in the wrong direction and the pistil is cast aside like a green splinter. Grrrr.

Before I put you off tomato breeding forever, I should mention that I have a preference for small tomatoes, which tend to come from small flowers. Many of the larger-fruited varieties have much sturdier blossoms which are relatively easy to work with. Ever wondered why large-fruited tomatoes are so popular among hobbyist breeders? Now you know.

Just to show that I can and do get successful hand-pollinations though, here is a product of my own fair wobbly hand.

Banana Legs x Green Tiger F1

It's an F1 hybrid of Banana Legs x Green Tiger, a cross I made in 2008. Let me admit now that I know very little about tomato genetics, and made this cross very much on a whim just to see what would happen. Banana Legs is an American variety derived from the breeding work of Tom Wagner, though it wasn't raised by Tom himself but selected from a batch of mixed seed bought from his TaterMater company in the 1980s. It's a long plum tomato with a bright banana yellow skin with silver-green stripes, and yellow flesh, and attractive lacy foliage. Green Tiger is something of an enigma, as I obtained it from a packet of Marks & Spencer's eating-tomatoes, and they claim it's exclusive to them (or it was, until me and dozens of other gardeners started saving and sharing its seeds). It has a dark olive green and red striped skin, dark red flesh, and is as round and shiny as a snooker ball, but with a better flavour. Intriguingly, the F1 is producing egg-shaped fruits, which are pretty much intermediate between the two parent fruit shapes.

My tomato experiments are always going to be limited though by the fact that I don't have the space to do it properly. It's all very well having this solitary F1 plant, as you don't need to grow many plants at the F1 stage. Next year when I come to plant the F2 I will have a problem, as I won't be able to grow more than three or four plants ... so it's pot luck whether I'll get any interesting phenotypes. That's fine though ... I have enough on my plate with the peas and potatoes, and can do without too many extra projects. I'd rather give away the F2 seed, if I can find anyone who wants some, so that those with more space and more tomato passion can make use of it.

Here's another tomato curiosity: variegated Green Zebra.

Green Zebra variegation
Variegated leaves on a Green Zebra tomato.

It would be nice if this was a heritable feature, but no such luck. It's a spontaneous somatic mutation - which is the posh way of saying that nature freaked out and made a cockup in the cell division, and the cockup then replicated itself, resulting in two different types of leaf tissue within the same leaf. As the cockup is in the cells of the leaf, and not encoded in the DNA, it won't be passed on to the plant's offspring. In fact this tomato is already reverting to normal fully green growth.

And funnily enough, there is a similar thing going on in one of my peas. This is a variegated form of the already lovely Buerre Cosse Rouge. Again, I'm pretty sure it's a somatic mutation and won't be passed on in the seeds.

Variegated pea leaves

Then there's my Wilfred Owen poppies, which were pretty much the first thing I wrote about when I started this blog in February 2006, and you can read the story of them if you're interested. The gist is that I collected wild poppy seeds from plants growing in a relic of a first world war trench on the Somme. The trench was, I believe, occupied by Wilfred Owen in January 1917 and his poem The Sentry was written about his experience in it. I've been growing the Somme poppies for many years in my garden, and they are rather lovely ... deep silky bright red with a distinctive black cross at the base, though they vary in how strongly the black cross is expressed.

Somme poppy
Somme poppy, with a partial black cross.

The native wild poppies of northern France probably haven't changed much since WW1, but during its tenure in my garden the Somme poppy has taken the opportunity to hybridise with some Mother of Pearl poppies I had growing elsewhere at one time. There's not a lot you can do about this; poppies are sluttily promiscuous and will cross over large distances. And I can't say it bothers me. I'm of the view that genes are the important thing, and outer appearance is secondary. I still get plenty of "true" Somme phenotypes every year, and additionally I get some beautiful variants like this.

Hybrid poppy
Natural hybrid between a Somme poppy and a garden variety. It has the perfect black basal cross of the Somme type with the pink radial stripes of Mother of Pearl.

And finally, a whinge.

When I moved into this house/garden, there was a trellis fence along the western boundary adjoining my main vegetable plot. The previous owner, who was also a keen vegetable gardener, had made a point of having a fence there which let full sunlight through to his vegetable plot. Well, my next door neighbours just took it upon themselves, without consulting me, to remove the trellis fence and replace it with solid 6ft panels. I can see why they didn't consult me. They knew very well I would object, on the grounds that I now have a permanent shadow along a sizeable strip of my vegetable plot. They didn't even do a tidy job ... I'm sure it looks immaculate on their side but they've lumbered me with scrappy bits of wooden battens with sharp nails sticking out of the wood. As they just went ahead and did it, the only recourse I have would be to try to force them legally to remove it. Do I want to get into legal shenanigans with people I have to live next to? No, not really. But all the same, I am well pissed off.

I get on fine with the neighbours and haven't had any dispute with them before, although our garden ideals are polar opposites. Their garden is polished and scrubbed with lawns as sterile as astroturf, and mine is a voluptuous muddle. That, it seems, is the reason for the fence - the missus got fed up with untidy things from my garden growing through the trellis. They have no knowledge of the work I do with my scruffbag plot; they just find it baffling that I grow vegetables for seed and don't eat them. Why save seeds when you can get them for 99p down B&Q? The concept of breeding new varieties and conserving heritage ones meets with blank incomprehension. It's just a different outlook on gardening, and neither of us appreciates the other's aesthetic or way of doing things. At least now they won't have to worry about my dandelion seeds contaminating their garden, and I won't have to worry about their chemical sprays contaminating mine. The privacy is also a blessing. But I'm still pissed off.

Taking a fence
Yes I know it's extremely childish, but it makes me feel better.

Thursday 1 July 2010

Hybridising peas: the video

Here's a demonstration of the method of hand pollinating peas for anyone who has 10 minutes and 39 seconds to spare. Sorry about the business with the cat, he distracted me and it wasn't really possible to edit it.